Mycobactericidal Activity Of 14 Mesoamerican Plant Extracts Popularly Used In Pulmonary Infections
DOI:
https://doi.org/10.54495/Rev.Cientifica.EdicionEspecial2008.184%20Keywords:
Mycrobactericidal activity, Mesoamerican plant , Pulmonary infectionsAbstract
Pulmonary tuberculosis is an infectious disease, caused in most cases by Mycobacterium tuberculosis, which is transmitted mainly by intimate interpersonal contact through inhalation of air with infected particles, it is rarely acquired by ingestion or skin wounds. Its onset is usually insidious, manifesting nonspecific symptoms such as malaise, weight loss, cough, and night sweats. Diagnosis is based on clinical, radiological and pathological manifestations, which are very nonspecific, and on confirmation by bacteriological and serological techniques. Its treatment is based on the association of drugs to avoid the selection of resistance and the need for prolonged treatments to be able to kill all the bacilli in their different growth phases.
In this study, the mycobactericidal activity of fourteen plants popularly used in pulmonary infections was determined. The ethanolic extracts used were Cecropia obtusifolia Bartolini (guaramo), Bursera simaruba (palo
jiote), Guazuma ulmifolia (caulote), Byrsonima crassifolia (nance), Hymenaea courbaril (guapinol), Sida rhombifolia (brush), Senecio salignus (chilca), Solatium torvum (dishwasher), Dorstenia contrajerva (contrahierba), Bougainvillea glaba (bouganvillea) , Mexican sambucasPresl. ( elder), Acacia farnesiana Willd. (subin), Litsea guatemalensis Mez. (laurel) and Liquidambar styraciflua L. (liquidambar).
The evaluation was carried out by means of the 3-(4.5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) bromide colorimetric bioassay, in which the change in color of the wells allows establishing the presence of the activity mycobactericide of the extracts, as well as the concentration in which it is presented. It was determined that none of the evaluated plants presented activity against M. tuberculosis ATCC H37Rv in a concentration range of 100 (μg/ml to 6.52 μg/ml. B. simaruba presented a minimum inhibitory concentration of 50 μg/ml and the extracts of 5. mexicana, C. obtusifolia, A. farnesiana, L. guatemalensis and L. styraciflua at 25 µg/mL against M. smegmatis ATCC 607.
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Copyright (c) 2008 S.P. Quiñónez, M. Samayoa, V. García, A. Cáceres, V. Matta
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