Effect of Five Native Plant Extracts Used as Adaptogens on In Vitro Lymphoproliferative Activity
DOI:
https://doi.org/10.54495/Rev.Cientifica.v16i1.236Keywords:
Effect, Native plant extracts, Adaptogens, Lymphoproliferative activity , In vitroAbstract
For the present study, five native plant extracts popularly used as adaptogens were used: Baccharis trinervis leaves (Herba de Santo Domingo), Byrsonima crassifolia leaves (Nance), Neurolaena lobata leaves (Tres Puntas) and rhizomes of Smilax domingensis and Smilax spinosa (Tarsaparilla), to demonstrate the possible immunomodulatory activity through lymphoproliferation in vitro.
All the ethanolic extracts were analyzed on a culture of lymphocytes in human peripheral blood using a concentration of 1mg/mL of extract. Initially, the lymphoproliferative assay was used to evaluate the activity of the extracts on lymphocytes, however it was not possible to validate it, so the manual method (direct lymphocyte count) was validated; the test was performed with this method.
Of all the extracts studied, those that presented immunosuppressive activity were the leaves of Byrsonima crassifolia and the rhizome of S. spinosa. The Minimum Effective Concentration (CEM) was determined for these extracts by comparing them to different concentrations: 500, 250, 125, 62.5, 31.2 and 15.6 μg/ml, the CEM for B. crassifolia was 62.5 μg/ml and for S. spinosa of 125 μg/ml.
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Copyright (c) 2003 N. Meza, M. Paredes, A. Cáceres
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