Comparison of macro and micro procedures to evaluate the inhibitory activity of pathogenic fungi and yeasts to man
DOI:
https://doi.org/10.54495/Rev.Cientifica.v18i1.170Keywords:
actividad inhibitoria, hongos, lev aduras patógenos al hombreAbstract
The screening of the antifungal activity allows to show in vitro how a plant extract inhibits the growth of a fungus under standard conditions. Currently, in the Faculty of Chemical Sciences and Pharmacy, the methodology of Macrodilution in Agar is used. which uses significant amounts of resources, materials and time. As part of the globalization processes. The National Committee for Clinical Laboratory Standardization (NCCLS) approved the microdilution plate technique as a standard for the measurement of antifungal activity, which, using a minimum amount of resources, provides results in less time. Despite its approval, this technique is not yet applied in Guatemala for screening. nor for the determination of minimum inhibitory concentration (C1M) of plant extracts, which is why the present study aimed to compare both techniques, based on the evaluation of 1 2 extracts of popular use against 4 ATCC strains of uniform levad fungi and 6 ATCC strains of filamentous fungi to determine if both techniques are comparable.
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Copyright (c) 2010 P.A. Flores, M.J. Guancín, C.M. Ozaeta, A. Cáceres, I. Gaitán
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