Inhibitory activity of acetylcholinesterase by extracts of 18 plant species native to Guatemala used in the treatment of nervous affections
DOI:
https://doi.org/10.54495/Rev.Cientifica.v23i1.108Keywords:
Lantana camara, Tagetes lucida, Valeriana prionophylla, Wigandia urens var. caracasana, Lippia graveolens, Chaptalia nutansAbstract
Neurodegenerative syndromes generate memory and behavior deterioration, usually in the elderly. The most common is Alzheimer´s disease, which is associated with decreased levels of acetylcholine (ACh) after an increase of acetylcholinesterase (AChE). Worldwide studies have described some secondary metabolites from plants that might inhibit the enzyme activity or retard neuronal deterioration. With the aim of finding inhibitors of AChE, activity was evaluated in extracts (dichloromethane and methanol) of 18 medicinal and aromatic plants used in Guatemala for nervous or memory disorders (Brugmansia candida, Cassia reticulata, Chaptalia nutans, Chiranthodendron pentadactylon, Dorstenia contrajerva, Erythrina berteroana, Lantana camara, Lippia graveolens, Petiveria alliacea, Phlebodium pseudoaureum, Pimenta dioica, Salvia microphylla, Solanum nigrescens, Tagetes lucida, Ternstroemia tepezapote, Valeriana prionophylla, Vernonia deppeana and Wigandia urens var. caracasana). AChE inhibition was assessed qualitatively by two bioautographic TLC assays and quantitatively by a kinetic microcolorimetric evaluation. The results demonstrated activity by bioautography in all species at screening concentration (5 µl), but when quantified by microcolorimetry no significant activity (>50%) was demonstrated at 1 mg/mL. Three of the species with shown activity (L. camara, T. lucida and V. prionophylla) were partitioned to see if the liquid:liquid partions presented more activity, but this did not increase the activity. Intermediate activity was also found in extracts from C. nutans, D. contrajerva var. houstoni, L. graveolens and W. urens var. caracasana. Studies will continue regarding the antioxidant activity in order to determine whether there might be some potential use.
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Copyright (c) 2013 D. Ortiz, A. Valdez, L. López, I. Gaitán, M. Paz, S. Cruz, L. Álvarez, Armando Cáceres
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