Comparison Of Gene Frequencies In Two Generations Of Triatoma Dimidiata, Using RAPDS- PCR Molecular Genetic Techniques
DOI:
https://doi.org/10.54495/Rev.Cientifica.EdicionEspecial2002.246Keywords:
Gene frequencies, Triatoma Dimidiata, RAPDS-PCR, Molecular Genetic TechniquesAbstract
Molecular biology has been applied to the study of population genetics and these techniques have recently been focused on triatomine research. In this investigation, the Random Amplification of Polymorphic DNA (RAPD's) technique was applied to deepen the knowledge of the reproductive and migratory patterns of Triatoma dimidiata due to the fact that the reinfestation of T. dimidiata has been detected in sprayed houses. Since T. dimidiata is the main vector of Chagas disease, it was proposed to better understand its reproductive and migratory patterns, since this could contribute to improving strategies for the control of this vector.
Two types of samples were mainly selected: control individuals, resulting from the cross between two known virgin parents and field samples in which the male parents were unknown. The field individuals were collected in the municipalities of Ixhuatán and Pueblo Nuevo Viñas of the Department of Santa Rosa.
Understanding the genetic process related to the copulation and fertilization of these triatomines allowed us to better focus the alternatives against the reinfestation of the sprayed houses. The results obtained suggest that the females of T. dimidiata copulate with several males. This was visually appreciated when plotting with dendrograms the data of the cluster analyzes carried out between the control individuals and the field individuals. It is possible that it is the males of Triatoma dimidiata that determine the population movements of this species. since they are induced to copulate with several females and that they have a greater ability to fly.
This is the first work that documents polyandry in Triatoma dimidiata and raises the preponderant role of the male of this species in population movements.
It is recommended to expand this type of study basing the analysis on a greater number of evaluated loci and to use alternative techniques to running the samples in agarose gels, which allow a better resolution of the banding pattern.
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Copyright (c) 2002 Maria Carlota Monroy, Juan José Chávez, Sergio Melgar, Antonieta Rodas, Silvia Bor, Regina Rosales, Eunice Enriquez, Patricia Dorn
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