Determination of the immunomodulatory activity of two extracts of the fungus Grifola Frondosa (Dicks.:Fr.) S.F. Gray, Through In Vitro Assays
DOI:
https://doi.org/10.54495/Rev.Cientifica.EdicionEspecial2009.175%20Keywords:
Immunomodulatory activity, Grifola Frondosa, Extracts, In vitroAbstract
The activity of two extracts of the edible fungus Grifola frondosa (Dicks.:Fr.) S.F. Gray, commonly known as maitake on the human immune system. The immunomodulatory activity of the aqueous and ethanolic extracts obtained from the fruiting body of the fungus on lymphocytes was studied by means of a lymphoproliferative assay and in vitro hemolytic assays were performed to test their influence on the classical and alternate pathways of the complement system.
The lymphoproliferation assay was performed by placing purified lymphocytes exposed to the extracts and cultured for final colorimetric evaluation with XTT, a tetrazolium salt that is reduced to a soluble, colored compound by mitochondrial enzyme activity present in living cells.
For the evaluation of the extracts on the complement system, hemolytic assays based on the rupture of erythrocytes by the membrane attack complex (MAC) generated after the activation of the complement system were used.
The absorbance of the hemoglobin released by the erythrocytes was the parameter for measuring the activity of the complement system. Both G. frondosa extracts showed lymphoproliferation-stimulating activity, with greater activity being observed in the ethanolic extract. The bioassays on the complement system demonstrated an inhibitory action in both extracts and on the two complement pathways, classical and alternate.
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Copyright (c) 2009 R. Lorenzana, A. Mazariegos, M. Paz, F. Sommerkamp
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