Determination of immunoglobulin A in human milk before and after pasteurization
DOI:
https://doi.org/10.54495/Rev.Cientifica.v24i1.100Keywords:
immunoglobulin A, human milk, pasteurization, milk bankAbstract
Secretory IgA is the most frequent immunoglobulin found in human secretions which acts as a humoral barrier in mucosal epithelium giving protection against infections produced by microorganisms. The objective of this study was to determinate how the concentration of IgA is affected by the process of pasteurization. We collected 37 samples of mature milk from moms who attended the Human Milk Bank at the National Hospital Pedro Bethancourt, in Antigua Guatemala. One aliquot of 3 mL was collected before pasteurization and another aliquot of 3 mL was collected after pasteurization from each sample. The analysis of IgA was made by immunoturbidimetry. The average concentration of IgA before pasteurization was 18.86 mg/dL and the average concentration of IgA after pasteurization was 9.94 mg/dL. The diminution of the IgA concentration was significant after pasteurization (p<0.0001). We observed a negative correlation between the concentration of IgA and Dornic acidity. There was a 48.28 % reduction of IgA original concentration but despite the reduction of IgA associated to the process of pasteurization of the human milk, samples still contain an average of 51.72% of the original concentration of this important immunologic protein which give protection to the infants that are fed with this milk.
Downloads
References
Akinbi, H., Meinzen-Der, J., Auer, C., Ma, Y., Pullum, D., Kusano, R., et al. (2010). Alterations in the Host Defense Properties of Human Milk Following Prolonged Storage or Pasteurization. Journal of Pediatric Gatroenterology and Nutrition, 51, 347-352. https://doi.org/10.1097/MPG.0b013e3181e07f0a DOI: https://doi.org/10.1097/MPG.0b013e3181e07f0a
APP. (2005). Breastfeeding and the use of human milk. Pediatrics, 115, 496–506. https://doi.org/10.1542/peds.2004-2491 DOI: https://doi.org/10.1542/peds.2004-2491
Arvola, M., Gustafsson, E., Svensson, L., Jansson, L., Holmdahl, R., Heyman, B., et al. (2000). Immunoglobulin-Secreting Cells of Maternal Origin Can Be Detected in B Cell-Deficient Mice. Biology of Reproduction, 63, 1817–1824. https://doi.org/10.1095/biolreprod63.6.1817 DOI: https://doi.org/10.1095/biolreprod63.6.1817
Bartolozo, E. Q., Candido, L. M., Colombo, A. O. & Santos, G. (2010). Protein supplementation effects on human milk protein and A immunoglobin concentrations. Revista do Instituto Adolfo Lutz, 69, 327-332. DOI: https://doi.org/10.53393/rial.2010.v69.32633
Braga, L. P. M. & Palhares, D. B. (2007). Effect of evaporation and pasterurization in the biochemical an immunological composition of human milk. Jornal de Pediatria (Rio J.), 83, 59-63. https://doi.org/10.2223/JPED.1578 DOI: https://doi.org/10.2223/JPED.1578
Hartmann, S. A., Wigdahl, B., Neely, E. B., Berlin, C. M., Schengrund, C., Lin, H. & Howett M. K. (2006). Biochemical Analysis of Human Milk Treated With Sodium Dodecyl Sulfate, an Alkyl Sulfate Microbicide That Inactivates Human Immunodeficiency Virus Type 1. Journal of Human Lactation, 22, 61- 73. https://doi.org/10.1177/0890334405280651 DOI: https://doi.org/10.1177/0890334405280651
Humbert, G., Guingamp, M. F. & Gaillard, J. L. (2006). The Clarifying Reagent, or how to make the analysis of milk and dairy products easier. Journal of Dairy Research, 73, 1–8. https://doi.org/10.1017/S0022029906001865 DOI: https://doi.org/10.1017/S0022029906001865
Kolbe, M. (2009). Implementación de buenas prácticas de manufactura y elaboración del plan de análisis de peligros y puntos críticos de control -haccp-en el banco de leche materna del Hospital Nacional Pedro de Bethancourt, San Felipe de Jesús Antigua Guatemala. Universidad Rafael Landivar. Guatemala.
Lawrence, R. A. (1999). Storage of human milk and the influence of procedures on immunological components of human milk. Acta Pediatrica, 88, 14-18. https://doi.org/10.1111/j.1651-2227.1999.tb01295.x DOI: https://doi.org/10.1111/j.1651-2227.1999.tb01295.x
Linares, S., Ortiz, K., Soto, M.A., Moreira, R., & Arroyo, G. (2013) [IgA concentrations in breast milk samples during the processing phases at the Human Milk Bank, National Hospital "Pedro de Bethancourt‖, Antigua Guatemala]. Información no publicada
Mestercky, J. & McGhee, J. R. (1987). Immunoglobulin A (IgA): molecular an celular interactons involved in IgA biosynthesis and immune responses. Advances in immunology, 40, 153. https://doi.org/10.1016/S0065-2776(08)60240-0 DOI: https://doi.org/10.1016/S0065-2776(08)60240-0
Novak, F. R. & Cordeiro, D. M. (2007). The correlation between aerobic mesophilic microorganism counts and Dornic acidity in expressed human breastmilk. Jornal de Pediatria, 83, 87-91. https://doi.org/10.2223/JPED.1589 DOI: https://doi.org/10.2223/JPED.1589
Ogundele, M. O. (2000). Techniques for the storage of human breast milk: implications for anti-microbial functions and safety of stored milk. European Journal of Pediatry, 159, 793–797. https://doi.org/10.1007/s004310000577 DOI: https://doi.org/10.1007/s004310000577
Permanyer, M., Castellote, C., Ramírez-Santana, C., Audí, C., Pérez-Cano, F. J., Castell, M., et al. (2010). Maintenance of breast milk immunoglobulin A after high-pressure processing. Journal of Dairy Science, 93, 877–883. https://doi.org/10.3168/jds.2009-2643 DOI: https://doi.org/10.3168/jds.2009-2643
Soto, M.A. y Moreira, R. (2007) Manual Técnico y Funciones del Banco de Leche Materna. Hospital Nacional Pedro de Bethancourt. Guatemala.
Tomicic, S., Johansson, G., Voor, T., Björksten, B. Böttcher, M. F. & Jenmalm, M. C. (2010). Breast Milk Cytokine and IgA Composition Differ in Estonian and Swedish Mothers—Relationship to Microbial Pressure and Infant Allergy. Pediatric Research, 68, 330-334. https://doi.org/10.1203/PDR.0b013e3181ee049d DOI: https://doi.org/10.1203/PDR.0b013e3181ee049d
Viazis, S., Farkas, B. E., Allen, J. C. (2011). Effects of High-Pressure Processing on Immunoglobulin A and Lysozyme Activity in Human Milk. Journal of human Lactation, 23, 253-261 DOI: https://doi.org/10.1177/0890334407303945
World Health Organization. (11/01/2011). Exclusive breastfeeding for six months best for babies everywhere. Disponible en: http://www.who.int/mediacentre/news/statements/2011/breastfeeding_20110115/en/index.html
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2014 K. Ortiz, R. Moreira, M. Soto, G. Arroyo
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
