Synthesis of polymer chitosan nanoparticles funtionalized with blackberry extracts(Rubus glaucus) and its preliminary evaluation as antimicrobial agents
DOI:
https://doi.org/10.54495/Rev.Cientifica.v22i1.126Keywords:
antimicrobial agents, polyphenols, bacteriostatic effect, chitosan, polimeric nanoparticlesAbstract
The aim of this study is to evaluate the bacteriostatic effect of functionalized chitosan nanoparticles with antioxidants from tropical fruit extracts.
In the synthesis and characterization of functionalized chitosan nanoparticles variables are treated in order to obtain concentrations and other parameters, which shows bacteriostatic effect on some species of pathogenic gram-negative ATCC bacterias: Escherichia coli, Serratia marcescens and Pseudomona aeroginosa, and gram-positives: Staphylococcus aureus, Bacillus subtilis and Enterococcus faecalis.
The hypothesis assumes that functionalized nanoparticles synthesized according to the ion gelation method using tripolyphosphate as crosslinking agent, with modifying agents from blackberry tropical fruit extracts (Rubus glaucus), containing antioxidants, expressed as gallic acid concentration, provides chitosan nanoparticles the ability to inhibit bacterial growth.
This research performed a preliminary evaluation of the polyphenol content and antimicrobial activity in conjunction with the nanoparticles against pathogens mentioned above. The evaluated fruits exhibit concentrations of 100 mg of gallic acid/100g and antimicrobial activity against the gram-positive S. aureus E. faecalis and B. subtilis, and the gram-negative S. marcescens determined by disc diffusion method showing an inhibition diameter between 10% and 60 % compared to positive control. These results show the potential of these nanoproducts as antimicrobial agents for use in the pharmaceutical industry.
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Copyright (c) 2012 M. Porras, S. Madrigal, J. Vega
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