Obtaining and purifying antigens for serological diagnosis of amoebiasis
DOI:
https://doi.org/10.54495/Rev.Cientifica.v9i2.383Keywords:
obtaining, purifying, antigens, serological diagnosis of amoebiasisAbstract
The document summarizes the tests carried out for the standardization of a serological method (Enzyme-linked immunosorbent assay, ELISA) for the diagnosis of amoebiasis, using antigen obtained from axenic culture of Entamoeba histolytica. The study was carried out in two phases, the first consisted of obtaining antigen; for this, the amoebas were harvested in culture flasks in the logarithmic phase of growth, thus obtaining a solution containing 5 ug/ul of amoebic antigen. In the second phase, the sensitivity and specificity of the antigen obtained was tested by confronting it with monoclonal and polyclonal antibodies by means of the ELISA technique. It was found that under these conditions the antigen was capable of detecting antibody titers up to 1:6400, using commercial conjugates at double the recommended dilution. The antigen was also tested against patient sera and commercial controls, and was simultaneously compared to a commercial ELISA method, where the same results were obtained by both methods. It is concluded that the antigen prepared in the laboratory gives good results. It is recommended that more tests be carried out in order to standardize the method for diagnostic purposes and seroepidemiological studies that provide a real framework for the prevalence of amoebiasis in Guatemala.
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References
Martínez Palomo A. The pathogenesis of amoebiasis. Parasitol. Tod. 1987:4:111-118, https://doi.org/10.1016/0169-4758(87)90048-2 DOI: https://doi.org/10.1016/0169-4758(87)90048-2
Trissl D. Inmunology of Entamoeba histolytica in human an animal hosts. Rev. Inf. Dis. 1 982;6:1 1 54-1183.
Joyce P, Ravdin J. Antigens of Entamoeba histolytica recognized by immune sera from liver abscess patients. Am J Trop Med & Hyg. 1 988;38:74-80, https://doi.org/10.4269/ajtmh.1988.38.74 DOI: https://doi.org/10.4269/ajtmh.1988.38.74
England PT. Sher A. The biology of parasitism; a molecular and immunological aproach. New York: Alan R. Liss Inc, Doc. tec. 1988. 554 p.
Diamond LS. Entamoaeba histolytica shaudinn;1 903: from xenic to axénic cultivation. J Protozool 1986;33:1-5, https://doi.org/10.1111/j.1550-7408.1986.tb05545.x DOI: https://doi.org/10.1111/j.1550-7408.1986.tb05545.x
De la Torre M. et al. Cultivos axénicos de Entamoeba histolytica. Arch of Inv Med (Mex) 1 971 ;2:1 65-172.
Collins WP. Alternative and complementary immunoassays. Folleto informativo. 1984.
Voller A. Bidwell DE. Heterogeneus enzime immunoasays (ELISA) difficulties encountered and ther resolution; Biology Prospective 5a. coloque international. Doc Tec. 1983; pp 217-222.
Strachan WD et al. Inmunological diferentiation of pathogenic isolates of Entamoeba histolytica. Lancet 1988;12:561-562, https://doi.org/10.1016/S0140-6736(88)91355-4 DOI: https://doi.org/10.1016/S0140-6736(88)91355-4
Robinson GL. The laboratoy diagnosis of human parasitic amoeba, Trans, of Roy. Soc. Trop. Med. & Hyg.,1982, 76:465-472
Grundy M . Prelyminary observations using a multy layer ELISA method for detecction of Entamoeba histolytica trophozoite antigens i n stool samples. Trans. Soc. Trop. Med. & Hyg., 1982; 76:396-400. DOI: https://doi.org/10.1016/0035-9203(82)90199-7
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Copyright (c) 1994 Jorge Rodolfo Pérez Folgar, Mariana Eugenia Paredes Sánchez, Rafael Armando Elgueta Spinola, Óscar Federico Nave Herrera, Maritza Martínez Garzaro
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