Preparation of Aspergillus species antigens and their use in the diagnosis of pulmonary pergillosis secondary to tuberculosis
DOI:
https://doi.org/10.54495/Rev.Cientifica.v2i2.475Keywords:
preparation, Aspergillus species antigens, Aspergillus species antigens use in diagnosis, diagnosis, pulmonary pergillosis secondary to tuberculosisAbstract
Aspergillosis is a non-contagious, sporadic, and cosmopolitan mycosis with diverse clinical manifestations, primarily respiratory, caused by different species of the genus Aspergillus. The pulmonary form is often a superimposed condition in patients with tuberculosis, silicosis, or an inadequate immune response related to corticosteroid or antibiotic therapy. The nonspecific clinical and radiological pulmonary manifestations of aspergillosis create diagnostic challenges. Immunological reactions often provide the first and only indication of a fungal infection, as histological evidence of the disease is very difficult to obtain. The cost of reagents for serological tests for the diagnosis of aspergillosis is high, and their acquisition in our setting is difficult. The present study aimed to demonstrate that in Guatemala it is possible to prepare Aspergillus antigens at low prices for use in diagnostic testing and immunodiffusion in populations with pulmonary conditions. The results reveal that the preparation of Aspergillus antigens is simple, and production costs are very low compared to the prices of commercial antigens. Furthermore, the specificity of the prepared antigens was evaluated against sera from patients with other fungal diseases and from apparently healthy individuals. No false-positive reactions were obtained. It is therefore concluded that the prepared antigens are reliable and specific for the diagnosis of aspergillosis. With the A. fumigatus antigen, it was possible to detect positive cases in a group of 103 patients diagnosed with pulmonary tuberculosis. From this, it is concluded that aspergillosis secondary to tuberculosis does exist in Guatemala, making it necessary to perform this test routinely in individuals who appear to have tuberculosis or other diseases that predispose to the acquisition of aspergillosis.
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Copyright (c) 1984 Lilian Ninette Guzmán Aguilar, Patricia Cáceres de Cifuentes
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