Preparation of Aspergillus species antigens and their use in the diagnosis of pulmonary pergillosis secondary to tuberculosis

Authors

  • Lilian Ninette Guzmán Aguilar Faculty of Chemical Sciences and Pharmacy
  • Patricia Cáceres de Cifuentes Faculty of Chemical Sciences and Pharmacy

DOI:

https://doi.org/10.54495/Rev.Cientifica.v2i2.475

Keywords:

preparation, Aspergillus species antigens, Aspergillus species antigens use in diagnosis, diagnosis, pulmonary pergillosis secondary to tuberculosis

Abstract

Aspergillosis is a non-contagious, sporadic, and cosmopolitan mycosis with diverse clinical manifestations, primarily respiratory, caused by different species of the genus Aspergillus. The pulmonary form is often a superimposed condition in patients with tuberculosis, silicosis, or an inadequate immune response related to corticosteroid or antibiotic therapy. The nonspecific clinical and radiological pulmonary manifestations of aspergillosis create diagnostic challenges. Immunological reactions often provide the first and only indication of a fungal infection, as histological evidence of the disease is very difficult to obtain. The cost of reagents for serological tests for the diagnosis of aspergillosis is high, and their acquisition in our setting is difficult. The present study aimed to demonstrate that in Guatemala it is possible to prepare Aspergillus antigens at low prices for use in diagnostic testing and immunodiffusion in populations with pulmonary conditions. The results reveal that the preparation of Aspergillus antigens is simple, and production costs are very low compared to the prices of commercial antigens. Furthermore, the specificity of the prepared antigens was evaluated against sera from patients with other fungal diseases and from apparently healthy individuals. No false-positive reactions were obtained. It is therefore concluded that the prepared antigens are reliable and specific for the diagnosis of aspergillosis. With the A. fumigatus antigen, it was possible to detect positive cases in a group of 103 patients diagnosed with pulmonary tuberculosis. From this, it is concluded that aspergillosis secondary to tuberculosis does exist in Guatemala, making it necessary to perform this test routinely in individuals who appear to have tuberculosis or other diseases that predispose to the acquisition of aspergillosis.

Downloads

Download data is not yet available.

References

Connant NF, Smith DT, Baker RD, Callaway JL. Micología Médica. 3 ed. Colchero F. trad. Interamericana. México, 1972. XI + 592 p (p. 292-310).

Hoehne J, Reed C, Dickie H. Aspergillosis broncho-pulmonary is not rare. Chest 1973; 63:177-181. https://doi.org/10.1378/chest.63.2.177

Kurup VP, Fink N, Barboriak JJ, Scribner G. The detection of circulating antibodies against antigens from three strains of AspergiHus fumigatus. Mykosen 1980;23(7); 368-372. https://doi.org/10.1111/j.1439-0507.1980.tb02620.x

Longbotton JL, Pepys J. Pulmonary aspergillosis: diagnostic and immunological significance of antigens and C substances in AspergiHus fumigatus. J Path Bact 1964;88:141-146. https://doi.org/10.1002/path.1700880119

Coleman RM, Kaufman L. Use of the ¡mmunodiffusion test in the serodiagnosis of aspergillosis Apll Microbiol 1972;23:301-310. https://doi.org/10.1128/am.23.2.301-308.1972

Drouhet E, Camey L, Segretain G. Valeur de l'immunoprecipitation et de l'immunofluorescence indirecte dans les aspergilloses bronchopulmonaires. Ann Inst Pasteur 1972;123:379-395.

Kim SJ, et al. Caracterizaron of antigens from Aspergillus fumigatus. V. Reactivity in ¡mmunodiffusión tests with serums from patients with aspergillosis caused by AspergiHus ftavus, A. niger and A. fumigatus. Am R.ev Res Dis 1980;122:647-650.

Negroni R, Robles AM, Galussio JC Estudio comparativo de las reacciones serológicas cuantitativas con un antígeno metabólico de AspergiHus fumigatus. Mycol Appl 1972,48 275-287. https://doi.org/10.1007/BF02052632

Reyes O. Determinación de precípitinas aspergieres, México Universídao Autónoma de Puebla (tesis de graduación Facultad de Farmacia) 1970. 32p.

Schaefer JC, Yu B.armstrong D. An Aspergillus immunooiffusión test in the early diagnosis of aspergillosis in adult leukemia patients. Am Rev Res Dis 1976; 113 325-329.

Yarzabal L, et al. Pruebas de ¡nmunoprecípitación en el diagnóstico de la aspergilosis. Rev. Inst Med Trop Sao Paulo 1973; 15( 1): 1 -9.

Harrel WK, et al. Procedural Manual for Production of Bacterial, Fungal and Farasitic Reagents. Biological Reagents Section, Center for Disease Control. Atlanta Ga. 1973.

Dubois M. et al. The Phenol-Sulfuric acid test for carbohydrates. Analytical Chem 1976; 28:350-356. https://doi.org/10.1021/ac60111a017

Palmer D, Cavallaro JJ, Kaufman L, Kaplan W. Sero-d¡agnosis of micotic diseases. Inmunology series No.8 Procedural Guide. U.S. Department of Health Education and Welfare, Public Health Service, Center for Disease Control. Atlanta Ga. 1975.

Fraser D. Ward J, Ajello L, Plikaytis B. La aspergilosis y otras micosis sistémicas. JAMA en Centro América 1980;9:669-675.

Osoagbaka OU AspergiHus and Candida species isolated from sputa of patients with bronchopulmonary disorders in Nigeria. Mykosen 1980;24t9) :547-551.

Published

1984-12-31

How to Cite

Guzmán Aguilar, L. N., & Cáceres de Cifuentes, P. (1984). Preparation of Aspergillus species antigens and their use in the diagnosis of pulmonary pergillosis secondary to tuberculosis. Revista Científica, 2(2), 22–26. https://doi.org/10.54495/Rev.Cientifica.v2i2.475

Issue

Section

Original Research Papers

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.