Preparation of Aspergillus species antigens and their use in the diagnosis of pulmonary pergillosis secondary to tuberculosis
DOI:
https://doi.org/10.54495/Rev.Cientifica.v2i2.475Keywords:
preparation, Aspergillus species antigens, Aspergillus species antigens use in diagnosis, diagnosis, pulmonary pergillosis secondary to tuberculosisAbstract
Aspergillosis is a non-contagious, sporadic, and cosmopolitan mycosis with diverse clinical manifestations, primarily respiratory, caused by different species of the genus Aspergillus. The pulmonary form is often a superimposed condition in patients with tuberculosis, silicosis, or an inadequate immune response related to corticosteroid or antibiotic therapy. The nonspecific clinical and radiological pulmonary manifestations of aspergillosis create diagnostic challenges. Immunological reactions often provide the first and only indication of a fungal infection, as histological evidence of the disease is very difficult to obtain. The cost of reagents for serological tests for the diagnosis of aspergillosis is high, and their acquisition in our setting is difficult. The present study aimed to demonstrate that in Guatemala it is possible to prepare Aspergillus antigens at low prices for use in diagnostic testing and immunodiffusion in populations with pulmonary conditions. The results reveal that the preparation of Aspergillus antigens is simple, and production costs are very low compared to the prices of commercial antigens. Furthermore, the specificity of the prepared antigens was evaluated against sera from patients with other fungal diseases and from apparently healthy individuals. No false-positive reactions were obtained. It is therefore concluded that the prepared antigens are reliable and specific for the diagnosis of aspergillosis. With the A. fumigatus antigen, it was possible to detect positive cases in a group of 103 patients diagnosed with pulmonary tuberculosis. From this, it is concluded that aspergillosis secondary to tuberculosis does exist in Guatemala, making it necessary to perform this test routinely in individuals who appear to have tuberculosis or other diseases that predispose to the acquisition of aspergillosis.
Downloads
References
Connant NF, Smith DT, Baker RD, Callaway JL. Micología Médica. 3 ed. Colchero F. trad. Interamericana. México, 1972. XI + 592 p (p. 292-310).
Hoehne J, Reed C, Dickie H. Aspergillosis broncho-pulmonary is not rare. Chest 1973; 63:177-181. https://doi.org/10.1378/chest.63.2.177
Kurup VP, Fink N, Barboriak JJ, Scribner G. The detection of circulating antibodies against antigens from three strains of AspergiHus fumigatus. Mykosen 1980;23(7); 368-372. https://doi.org/10.1111/j.1439-0507.1980.tb02620.x
Longbotton JL, Pepys J. Pulmonary aspergillosis: diagnostic and immunological significance of antigens and C substances in AspergiHus fumigatus. J Path Bact 1964;88:141-146. https://doi.org/10.1002/path.1700880119
Coleman RM, Kaufman L. Use of the ¡mmunodiffusion test in the serodiagnosis of aspergillosis Apll Microbiol 1972;23:301-310. https://doi.org/10.1128/am.23.2.301-308.1972
Drouhet E, Camey L, Segretain G. Valeur de l'immunoprecipitation et de l'immunofluorescence indirecte dans les aspergilloses bronchopulmonaires. Ann Inst Pasteur 1972;123:379-395.
Kim SJ, et al. Caracterizaron of antigens from Aspergillus fumigatus. V. Reactivity in ¡mmunodiffusión tests with serums from patients with aspergillosis caused by AspergiHus ftavus, A. niger and A. fumigatus. Am R.ev Res Dis 1980;122:647-650.
Negroni R, Robles AM, Galussio JC Estudio comparativo de las reacciones serológicas cuantitativas con un antígeno metabólico de AspergiHus fumigatus. Mycol Appl 1972,48 275-287. https://doi.org/10.1007/BF02052632
Reyes O. Determinación de precípitinas aspergieres, México Universídao Autónoma de Puebla (tesis de graduación Facultad de Farmacia) 1970. 32p.
Schaefer JC, Yu B.armstrong D. An Aspergillus immunooiffusión test in the early diagnosis of aspergillosis in adult leukemia patients. Am Rev Res Dis 1976; 113 325-329.
Yarzabal L, et al. Pruebas de ¡nmunoprecípitación en el diagnóstico de la aspergilosis. Rev. Inst Med Trop Sao Paulo 1973; 15( 1): 1 -9.
Harrel WK, et al. Procedural Manual for Production of Bacterial, Fungal and Farasitic Reagents. Biological Reagents Section, Center for Disease Control. Atlanta Ga. 1973.
Dubois M. et al. The Phenol-Sulfuric acid test for carbohydrates. Analytical Chem 1976; 28:350-356. https://doi.org/10.1021/ac60111a017
Palmer D, Cavallaro JJ, Kaufman L, Kaplan W. Sero-d¡agnosis of micotic diseases. Inmunology series No.8 Procedural Guide. U.S. Department of Health Education and Welfare, Public Health Service, Center for Disease Control. Atlanta Ga. 1975.
Fraser D. Ward J, Ajello L, Plikaytis B. La aspergilosis y otras micosis sistémicas. JAMA en Centro América 1980;9:669-675.
Osoagbaka OU AspergiHus and Candida species isolated from sputa of patients with bronchopulmonary disorders in Nigeria. Mykosen 1980;24t9) :547-551.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 1984 Lilian Ninette Guzmán Aguilar, Patricia Cáceres de Cifuentes

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.