Determination of the presence of diethylstilbestrol in beef for human consumption.

Authors

  • Sandra Lisseth Secam Faculty of Chemical Sciences and Pharmacy
  • Julia Alicia Amado Zeissig Faculty of Chemical Sciences and Pharmacy

DOI:

https://doi.org/10.54495/Rev.Cientifica.v6i1.447

Keywords:

determination, presence of diethylstilbestrol, beef, human consumption

Abstract

The present study was conducted to investigate residues of the estrogenic substance diethylstilbestrol in beef consumed by the population of Guatemala City. To carry out this study, 57 samples were analyzed between January and June 1986, from slaughterhouses that supply meat to the city. The tissue used was liver, and the analytical method used was the one recommended by the United States Department of Agriculture, which had been previously tested and conditioned for this purpose. The detection limit obtained was 2-0 micrograms of diethylstilbestrol per kilogram of tissue. In conclusion, none of the samples analyzed yielded positive results for diethylstilbestrol residues. Therefore, it can be stated that the beef from these slaughterhouses during the study period was free of this substance. This suggests that the use of diethylstilbestrol is limited and does not present a major problem.

Downloads

Download data is not yet available.

References

Litter M. Farmacología. 6 ed. Argentina: El Ateneo, 1980. XIII + 1953 p. (p. 1242-1256).

Preston R L. Reduction of plasma urea-N by dietyIstilbestrol in ruminants. Proc Soc Exp Biol Med 1968; 129:250-56. https://doi.org/10.3181/00379727-129-33297 DOI: https://doi.org/10.3181/00379727-129-33297

Trenkle A. The anabolic effect of estrogens on nitrogen metabolism of growing and finishing cattle and sheep. p. 79-99 (In Lu F C, Rendel J, eds. Anabolic Agents in animal production. USA: Georg Thieme Publishers Stuttgart. Suppl vol V, 1976).

Clegg M T, Colé H H. The action of slilbestrol on the growth response in ruminants. J Animal Sci 1954; 13:108-130. https://doi.org/10.2527/jas1954.131108x DOI: https://doi.org/10.2527/jas1954.131108x

Noller KL, Fish Cr. DiethyIstilbestrol Usage: Its interesting past, important present and questionable future. Med Clinics North Am 1974; 58:793-810. https://doi.org/10.1016/S0025-7125(16)32122-8 DOI: https://doi.org/10.1016/S0025-7125(16)32122-8

McLachlan JA. Prenatal exposure to diethylstilbestrol in mice; Toxicological studies. J Toxicol Environ Health 1977;2:527-37. https://doi.org/10.1080/15287397709529453 DOI: https://doi.org/10.1080/15287397709529453

Vorhers H, et al. Teratogenesis and carcinogenesis in rat offspring ethylstilbestrol. Biochem Pharmacol 1979; 28:1865-77. https://doi.org/10.1016/0006-2952(79)90638-5 DOI: https://doi.org/10.1016/0006-2952(79)90638-5

Nomura T, Masuda M. Carcinogenic and teratogenic activities of diethylstilbestrol in mice. Life Sci 1980; 26:1955-62. https://doi.org/10.1016/0024-3205(80)90626-8 DOI: https://doi.org/10.1016/0024-3205(80)90626-8

Ird EA, Smirnoval IO, Byull E. Embryogenesis and early postnatal development of rats under transplacental administration of sex hormones. Biol Med 1980; 90:607-9. https://doi.org/10.1007/BF00834106 DOI: https://doi.org/10.1007/BF00834106

Preston RL. Biological responses to estrogen additives in meat producing cattle and lambs. J Anim Sci 1975; 41:1414-1430. https://doi.org/10.2527/jas1975.4151414x DOI: https://doi.org/10.2527/jas1975.4151414x

Umberger EJ, et al. Chemical determination of diethylstilbestrol residues in the tissues of treated chickens J Assoc Off Anal Chem 1963; 46:471-479. https://doi.org/10.1093/jaoac/46.3.471 DOI: https://doi.org/10.1093/jaoac/46.3.471

Donoho AL, et al. Gas chromatographic determination of diethylstilbestrol and its glucuronide in cattle tissues J Assoc Off Anal Chem 1973; 56:785-92. https://doi.org/10.1093/jaoac/56.4.785 DOI: https://doi.org/10.1093/jaoac/56.4.785

Day EW. Jr, Vantta LE, Sieck RF. The confirmation of diethylstilbestrol residues in beef liverby gas chromatography-mass spectrometry. J Assoc Off Anal Chem 1975;58:520-24. https://doi.org/10.1093/jaoac/58.3.520 DOI: https://doi.org/10.1093/jaoac/58.3.520

Hoffman B, Laschütza W. Radioinmunoassay for determining diethylstilbestrol in blood plasma and edible parts of cattle. Archiv für Lebensmittelhygiene 1980; 31:105-111.

Karg H, Vogt K. Control of hormone treatment in animals and residues in meat regulatory aspects and approches in methodology. J Assoc Off Anal Chem 1978;61:1201-1208. https://doi.org/10.1093/jaoac/61.5.1201 DOI: https://doi.org/10.1093/jaoac/61.5.1201

Organización de las Naciones Unidas para la Agricultura. Orientaciones para el establecimiento o el fortalecimiento de programas nacionales de vigilancia de la contaminación de los alimentos. Ginebra: OMS, 1979 105 p. (p. 39).

Libby JU. Meat hygiene. 4 ed. Philadelphia: Lea & Feberger 1975. XII + 658 p (p. 476-77).

Donoho AL, et al. A sensitive gas chromatographic determination of diethylstilbestrol in animal feeds. J Assoc Off Anal Chem 1971; 54:75-79. https://doi.org/10.1093/jaoac/54.1.75 DOI: https://doi.org/10.1093/jaoac/54.1.75

Gearien JE, Grabowski BF. Mehods of drug analysis. USA: Lea & Feberger, 1969. IX + 281 p (p. 76-77).

Smith WG, McNeil EE. Simple method for routine detection of residues of diethulstilbestrol (DES) in meat contaminated at levels as low as one part per billion. Anal Chem 1972; 44: 1084-87. https://doi.org/10.1021/ac60314a034 DOI: https://doi.org/10.1021/ac60314a034

Ponder C. Fluorometric determination and thin layer chromatographic identification of diethylstilbestrol in beef liver. J Assoc Off Anal Chem. 1974; 57:919-923. https://doi.org/10.1093/jaoac/57.4.919 DOI: https://doi.org/10.1093/jaoac/57.4.919

Ponder C. Fluorometric determination of diethulstilbestrol at low levels in animal feeds. J Assoc Off Anal Chem. 1974;50:987-991. https://doi.org/10.1093/jaoac/57.4.987 DOI: https://doi.org/10.1093/jaoac/57.4.987

Jare H, Ruttner O, Krocza W. The determination of estrogenic substances during routing examination of animals for the residues. Fleeschwirtschaft 1976; 56:1326-8.

Ryan JJ, Pilón JC. Chemical confirmation of diethylstilbestrol residues in beef livers. J Assoc Off Anal Chem 1976;59:817-820. https://doi.org/10.1093/jaoac/59.4.817 DOI: https://doi.org/10.1093/jaoac/59.4.817

Kohrman KA, MacGee J. Simple and rapid gas-liquid chromatographic determination of diethylstilbestrol in biological specimens. J Assoc Off Anal Chem 1977; 60:5-8. https://doi.org/10.1093/jaoac/60.1.5 DOI: https://doi.org/10.1093/jaoac/60.1.5

Hoffman B. Use of radioinmunoassay for monitoring hormonal residues in edible animal products. J Assoc Off Anal Chem 1978; 61:1263-73. https://doi.org/10.1093/jaoac/61.5.1263 DOI: https://doi.org/10.1093/jaoac/61.5.1263

Rumsey TS, Kozad AS. Analysis by geas-liquid and thin layer chromatography of residual (14C) diethylstilbestrol ear implants.

Sphon JA. Use of mass spectrometry for confirmation of animal durg residues. J Assoc Off Anal Chem 1978: 61: 1247-52. https://doi.org/10.1093/jaoac/61.5.1247 DOI: https://doi.org/10.1093/jaoac/61.5.1247

Vereenfachtes. Simplified extraction and purification procedure for radioimmunological assay of diethylstilbestrol in meat, liver and kidney. Archiv für Lebensmittelhygiene 1980; 31: 1138-41.

Moretti G, Cavina G, Pompi U. Identification of DES residues in edible animal tissues by means of mass spectrometry. Annali dell'lnst Sup di Sanita 1982; 16:831-35.

Tirpenou AE, Kilikidis SD, Kamarianos AP. Modified method for electrón capture gas-liquid chromatographic determination of diethylstilbestrol in urine of fattened bulls. J Assoc Off Anal Chem 1983; 66:1230-33. DOI: https://doi.org/10.1093/jaoac/66.5.1230

Published

1988-12-31

How to Cite

Lisseth Secam, S., & Amado Zeissig, J. A. (1988). Determination of the presence of diethylstilbestrol in beef for human consumption. Revista Científica, 6(1), 22–29. https://doi.org/10.54495/Rev.Cientifica.v6i1.447

Issue

Section

Original Research Papers

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.