Optimization of a Method for the Determination of the Carotenoids Profile in Fruits and Vegetables by High Resolution Liquid Chromatography (HPLC)
DOI:
https://doi.org/10.54495/Rev.Cientifica.v14i1.261Keywords:
Optimization, Determination of Carotenoid Profile, Fruits and Vegetables , High Resolution Liquid Chromatography (HPLC)Abstract
Carotenoids are fat-soluble, highly unsaturated compounds found in yellow and orange vegetables and green leaves. Its best known functions of human importance are the provitamin A activity of some of them, and their antioxidant properties. This last property has been associated with the prevention of some types of cancer. Due to the special characteristics of carotenoids, the methods that have been proposed for their analysis are long and expensive. In addition, they frequently have errors that lead to false results, both qualitatively and quantitatively. Another problem that arises when working with carotenoids is that of papones, which are not very commercially accessible and that after preparation degrade quickly, In recent years, the study of carotenoids has gained importance due to the different functions they have. in the body, and in some cases, by its industrial application. Therefore, it is necessary to propose a method that can quantify the main carotenoids found in vegetables and fruits quickly and at a very low cost. In this work, it is intended to isolate carotenoid patterns and set up a high-performance liquid chromatography (HPLC) method, which allows the rapid quantification of these components. Said method will be validated according to the parameters established for the case: recovery percentage, linearity, detection and quantification limit and reproducibility.Downloads
References
Adewusi.S. R.A. y Howar Bradbury. 1993 . Carotenoids in Cassava: Comparison of Open-Co lumn and HPLC Methods of Analysis. J. Sci. Food Agrie. 62:375-383, https://doi.org/10.1002/jsfa.2740620411 DOI: https://doi.org/10.1002/jsfa.2740620411
Craft, N. E. 1992. Carotenoid Re vers ed-Ph as e High-Performance Liquid Chromatography Methods: Reference Compendium. En Methods in Enzymology. Vol. 213. Pp 185-205, https://doi.org/10.1016/0076-6879(92)13121-D DOI: https://doi.org/10.1016/0076-6879(92)13121-D
Davies, B. H. 1976. Carotenoids. En Chemistry and Biochemistry of Plant Pigments. 2ed. London, Academic Press. Vol. 2. Pp 38-165.
Epler, K. L.C. Sander. R.G. Ziegler, S.A. Wise y N.E. Craft. 1992. Evalution of reversed-phase liquid
chomatographic columns for recovery and selectivity of selected carotenoids. J. Chromatogr 595:89-101, https://doi.org/10.1016/0021-9673(92)85149-N DOI: https://doi.org/10.1016/0021-9673(92)85149-N
Hart, D . y K . J. Scott. 1995. Development and evaluation of an HPLC method for the analysis of carotenoids in foods, and the measurement of the carotenoid content of vegetables and fruit commonly consumed in the UK. Food Chem. 54: DOI: https://doi.org/10.1016/0308-8146(95)92669-B
-111, https://doi.org/10.1016/0308-8146(95)92669-B DOI: https://doi.org/10.1016/0308-8146(95)92669-B
Latinfoods. 1 986. Memorias de la primera reunión sobre tablas de composición de alimentos. Latinfoods, 11-14 Nov. Arch. Latinoamer, Nutr, 37 (4): 609-810,
Mangles, A. R., et al. 1993. Carotenoid content of fruits and vegetables: An evalution of analytic data. J . A m . Diet. Assoc. 93: 284 -296, https://doi.org/10.1016/0002-8223(93)91553-3 DOI: https://doi.org/10.1016/0002-8223(93)91553-3
Official Methods of Analysis of the AOAC. 1984. 14 ed. Arlintong, Association of Official Analytical Chemists. Pp 834-835.
Parker, G. A. 1991. Validation in the Florida Department of Agriculture and Consumer Servicies’ Chemical Residue Laboratory. J. Assoc. Of. Anal. Chem. 74(5): 868-872, https://doi.org/10.1093/jaoac/74.5.868 DOI: https://doi.org/10.1093/jaoac/74.5.868
Rodríguez- Amaya, D. B, 1 989. Critical Review of Provitamin A Determination in Plant Foods. J. Micronutrient Anal. 5: 191-225.
Rodriguez-Amaya, D. B. y Amaya-Farfán, J. 1992 Estado actual de los métodos analíticos para determinar provitamina A, Arch. Latinoamer. Nutr. 42(2): 180-191.
Rodriguez-Amaya, D., M. Kimura, H.T. Godoy y H. K. Arima. 1988. A s s e s m e n t o f Provitamin A Determination by Open Column Cromatography/VisibleAbsorption S p ectrophotometry. J. Chromatogr. Sci. 26(12): 624-629, https://doi.org/10.1093/chromsci/26.12.624 DOI: https://doi.org/10.1093/chromsci/26.12.624
Scott, K . J. 1992 Observations on some of the problems associated with the analysis of carotenoids in foods by HPLC. Food chem. 45: 357-364, https://doi.org/10.1016/0308-8146(92)90036-2 DOI: https://doi.org/10.1016/0308-8146(92)90036-2
Scott, K. J, y D. J. Hart. 1993. Further observations on problems associated with the analysis of carotenoids by HPLC — 2: Column temperature. Food Chem. 47: 403-405, https://doi.org/10.1016/0308-8146(93)90186-J DOI: https://doi.org/10.1016/0308-8146(93)90186-J
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