Identification of Families of Secondary Metabolites in Myrica Cerifera.

Abstract

The present work was carried out with the objective of implementing methodologies to determine the presence of families of secondary metabolites; that are viable and feasible in our environment, taking into consideration the extraction technique used to obtain the extracts, comparing the extracts obtained by leaching (cold agitation) and the extracts obtained by Soxleth extraction (hot). For this purpose, the determination of secondary metabolites in the Myrica cerifera plant was carried out, using qualitative chemical techniques in three extracts using different solvents, which were obtained by a group of researchers at the Engineering Research Center of the University of San Carlos of Guatemala. ; The first stage of the screening consisted of performing 6 extractions with three different solvents and two different types of extractive techniques.

For the leaching technique, 60 gr. of the fruit of the Myrtle (Myrica cerifera) were placed in three Erlenmeyer flasks with a capacity of 250 ml and 200 ml of the extracting solvent were added
(hexane, methanol! and water) in this case each Erlenmeyer flask had a different solvent; was kept under cold agitation with a mechanical magnet (leaching) for 72 hours, replacing the
volume of solvent every 24 hours, the replaced solvent was collected. For the preparation of the hot extract, 10 gr. from the fruit and were divided into three portions: each of the portions was placed in a Soxleth extractor thimble. previously dry and tared; 100 ml of solvent were added to the armed Soxleth system, using hexane, methanol and water, each one separately. Each system was applied at 40, 60, 100ºC respectively for 24 continuous hours under reflux, collecting the extract at the end of this period.