Detection of β-lactamase genes blaTEM, blaSHV and blaCTX-M in community isolates of Escherichia coli
DOI:
https://doi.org/10.54495/Rev.Cientifica.v28i2.56Keywords:
extended spectrum β-lactamases (ESBL), community isolates, Escherichia coli, TEM, SHV, CTX - MAbstract
Globally, resistance to antibiotics is a public health problem, both in the hospital and in the community environment. e production of β-lactamases is the main mechanism of resistance in enterobacteria and usually the cause of resistance are the enzymes to the families TEM, SHV and CTX-M. The principal aim of this study was to detect - β-lactamase genes blaTEM, blaSHV and blaCTX-M in community strains of ESBL-producing Escherichia coli isolated from urine cultures of patients attended in the Laboratorio Clínico Popular of the Universidad de San Carlos de Guatemala in 2016. At least, one of the genes was detected in 90% of the 79 isolationses and in 53.2% of the isolations the three genes were detected. The frequency was 57% for blaCTX-M, 84% for blaSHV and 85% for blaTEM. e detection of the genes coding for TEM-1, SHV-11, CTX-M15 and CTX-M55 represent the first molecular characterization of ESBL-producing E. coli isolations in Guatemala and it is important to understand the spread of these strains at the community environment. The ESBL-producing E. coli isolations showed a high resistance to ciprofloxacin and trimethoprim sulfamethoxazole (78%) and low resistance levels for fosfomycin (2.5%) and nitrofurantoin (7.6%). The 11.39% of the strains showed resistance to a group of non-beta-lactam antibiotics. It is important to establish an active surveillance for these antibiotics in community strains because they are the first line treatment for ESBL producing strains.
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