Preparation, evaluation and standardization of Coccidioides immitis antigen for serological diagnosis by immunodiffusion
DOI:
https://doi.org/10.54495/Rev.Cientifica.v3i1.465Keywords:
preparation, evaluation, standardization, Coccidioides immitis antigen, serological diagnosis, immunodiffusionAbstract
Extensive studies conducted by various authors have shown that pulmonary mycoses such as coccidioidomycosis occur quite frequently in the Americas.
Coccidioidomycosis is an infectious disease caused by Coccidioides immitis that presents a wide variety of clinical manifestations. The exact extent of this problem in Guatemala has not yet been determined since its diagnosis depends on the isolation and identification of the infecting fungus, which is not always possible.
Immunological methods provide the primary evidence of a fungal infection. It has been observed that in cases of coccidioidomycosis, immunodiffusion (ID) is a rapid and reliable serological method for the detection of humoral antibodies as an indication of active disease. However, due to the difficult and high cost of reagents, it is becoming increasingly necessary to produce our own reagents locally. The primary objective of this study was the preparation, evaluation, and standardization of C. immitis antigens for use in serological immunodiffusion tests for the diagnosis of pulmonary coccidioidomycosis in a Guatemalan population with pulmonary diseases. One positive case was found in a group of 100 patients.
The mycelial antigen was compared with the metabolic (soluble) antigen, confirming that the metabolic antigen is not useful for the immunodiffusion (ID) test, as no precipitation bands were obtained.
The specificity of one of the prepared antigens was also evaluated in the ID test using serum from patients with other fungal diseases and from apparently healthy individuals. No false-positive reactions were found. Therefore, it is considered a reliable, specific, and useful reagent for the diagnosis of coccidioidomycosis.
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References
Kaufman L. Serology: its valué in the diagnosis of coccidioidomyeosis, criptococosis and histoplasmosis, PAHO. 1970; 205: 96-100.
Connant N, Smith D, Baker R, and Callaway J. Micología, 3 ed. Colchero F. trad. México: Interamericana, 1972. XI 4- 592p (p 134-160).
Emmons C W, Binford C, and Utz J. Medical Micology 3 ed. Philadelphia: Lea and Fabiger, 1977. V 4- 508p (p 207 - 229).
Mayorga R, Espinoza H. Coccidioidomycosis in México and Central América. Mycopathol. Mycol. appl. 1970; 41: 13-23. https://doi.org/10.1007/BF02051480
Andrade C. Investigación de la Coccidioidomicosis en la capital de Guatemala por medio de la intradermoreacción a la coccidioidina: Universidad de San Carlos de Guatemala, (Tesis de graduación. Facultad de Ciencias Médicas) 1945. 32p.
Mayorga R. Coccidioidomycosis in Central América. University of Arizona Press, 1967; 8: 287-291.
Ajello L. Coccidioides immitis, isolation procedures and diagnostic criterio. U.S. Department of Health, Education and Welfare, Public Health Service, Atlanta Ga. 1953; 575: 47-52. https://doi.org/10.1080/00362176685190231
Ajello L, Grecelias G, Hugenholtz PG, Hall Lb. Recorvery of Coccidioides immitis from the air. J. Int. Coc. Hum. Anim. Mycol 1965; 4: 92-95.
Kaufman L. Serodiagnostic of Fundal deseases. In Rose V. R. Friedman H. eds Manual of Clinical inmunology. Washington, D.C. Am. Soc. Microbiol. 1976; 363-381 p.
Huppert M. Serology of Coccidioidomycosis. Rev. Med. Vet. Mycol. 1970; 41: 107-113. https://doi.org/10.1007/BF02051487
Harrel W. R. et al. Procedural Manual for Production of Bacterial, Fungal and Parasitic Reagents. Biological Reagents Section, Centre for Disease Control, Atlanta G.A. 1973.
Dubois M. et al. The Phenol-Sulfuric acid test for Carbohidratos. Analytical Chem. 1976; 28; 350-356.
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Copyright (c) 1985 Angela Victoria Pérez Reyes, Patricia Cáceres
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