Guatemalan anamorphic fungi and their potential use in production of α-amylases using rice husk as a substrate
DOI:
https://doi.org/10.54495/Rev.Cientifica.v29i2.39Keywords:
fungal enzymes, asexual fungi, Aspergillus sp., Beltrania rhombicaAbstract
Agroindustrial wastes are generated in large quantities and in most cases deposited in landfills as waste. These lignocellulosic residues can be raw material or substrate for anamorphic fungi, which through fermentation processes can produce biofuels, enzymes, vitamins, antioxidants, animal feed, antibiotics and other chemical products. In this study, the α-amylase production capacity of 20 native strains of anamorphic fungi from the fungal strain collection of Departamento de Microbiología, Facultad de Ciencias Químicas y Farmacia, USAC was determined through solid state fermentation, using rice husk as a substrate. The extraction of the enzymes was carried out by microfiltration and the amylolytic activity was measured by spectrophotometry. Of the strains evaluated, it was found that the amylases of Aspergillus sp. SL15319 showed the highest mean activity (standard deviation), both free, 930.26 (1.56) UA/ dl, and immobilized, 900.34 (3.21) UA/dl, followed by those of Beltrania rhombica, 905.02 (10.72) and 879.07 (3.87) UA/ dl and Aspergillus sp. SL15119 907.46 (5.17) and 875.95 (9.39) UA/dl (p < .05). The importance of this study lies in making known the potential of native anamorphic fungi in Guatemala for the use of agro industrial waste as a raw material for the production of substances of use to humans, and in reducing the pollutant load that is discharged into the environment.
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Copyright (c) 2020 Ricardo Figueroa Ceballos, María del Carmen Bran González, Osberth Morales Esquivel y Gustavo Adolfo Álvarez Valenzuela
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