Use of chicken bone for enzyme immobilization: I. Immobilization of papain
DOI:
https://doi.org/10.54495/Rev.Cientifica.v11i1.371Keywords:
utilization, chicken bone, Inmovilización de papaína, immobilization of papainAbstract
Granular chicken bone is a material that allows the immobilization of enzymes by adsorption. In the present study, the use of chicken bone for papain immobilization was tested. Granular chicken bone was produced locally on a small scale from raw chicken bone; one kg of this yielded 109 gr. of dry material of different particle sizes (mesh 12-60) and a production cost of approximately Q.200.00/kg. Chicken bone with mesh 14-20 particles was tested to determine its capacity and efficiency as a support for papain immobilization. It showed an immobilization capacity of 10.84 ± 0.36 mg of papain/gr. dry weight (x ± n = 3), the immobilized papain was active and exhibited 26.9 ± 5 percent (x ± n = 3) of its native enzyme activity. Stability tests indicated that papain immobilized on bone is reusable, retaining 59-67 percent (n=3) of the activity exhibited in the first test in a second test. The results demonstrated that local production of granular chicken bone and its use as a material for papain immobilization is possible.
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Copyright (c) 1996 Rubén D. Miranda Velásquez, Carmen G. Castillo Narvaez, Magda T. Flores López, Nancy E. López Estrada
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