Isolation, verification and identification of the active ingredient responsible for the antifungal activity of Bixa orellana (Achiote) leaves.
DOI:
https://doi.org/10.54495/Rev.Cientifica.v13i1.328Keywords:
isolation, verification, identification, active ingredient responsible for the antifungal activity, Bixa orellana (Achiote) leavesAbstract
Four flanovoid-type compounds were isolated from the active antifungal fraction of Bixa orellana leaves. These flavonoids were identified as; Compound 1: Quercetin-3-O-rutinoside Compound 2: Quercetin-3-O-robinobioside Compound 3: lsoramnetin-3-O-rutinoside Compound 4: lsoramnetinO-3-O-robinobioside. The isolation of the compounds present in the leaves of B. orellana was achieved after phytochemical fractionation in an open column with Sephadex LH-20 as a stationary phase and methanol as an eluent, followed by reversed-phase high-performance liquid chromatography (HPLC) in a WATERS p-Bondapak C-18 column and a 50:50 methanol-water mixture as eluent. The structural elucidation of the compounds was carried out by means of 13^C and 1^H Nuclear Magnetic Resonance (NMR) and Fast Atom Bombardment Mass Spectrometry (FABMS); thanks to the support of Dr. Luca Rastrelli from the Faculty of Pharmacy, University of Salerno, Italy. The isolation and identification of the compounds responsible for the antifungal activity of the leaves of B. orellana (general objective of the research) was achieved by obtaining the structures and names of the compounds present in the fraction with positive antifungal activity. Wet chemical tests and analysis by thin layer chromatography (TLC) allowed us to establish the probability that flavonoid-type compounds were present in the active fraction. Analysis by 13C and 1H NMR, Mass Spectrometry and Gas Chromatography confirmed that the compounds present in the active fraction were flavonoids.Downloads
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Copyright (c) 2000 Johanna Chew de Campos, Aura Padilla Arreaga
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